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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-62101 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-62101, RRID:AB_958768
- Product name
- Sheep Polyclonal 14-3-3 zeta/beta Antibody
- Antibody type
- Polyclonal
- Description
- IgG purified. NB100-62101 recognizes the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit, Sheep
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: 14-3-3 zeta/beta [p Ser185] Antibody [NB100-62101] - WB with Sheep anti-14-3-3 (pSER185) antibody (AHP1049) Lane 1, 2ug recombinant 14-3-3 beta(2), 2ug recombinant 14-3-3 zeta(3), sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185 The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated.
