sc-1019

antibody from Santa Cruz Biotechnology

Targeting: YWHAZ 14-3-3-zeta, KCIP-1, YWHAD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-1019

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-1019, RRID:AB_2218378

Product name

Anti-YWHAZ

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.

Okamoto Y, Shirakashi Y, Ihara M, Urushitani M, Oono M, Kawamoto Y, Yamashita H, Shimohama S, Kato S, Hirano A, Tomimoto H, Ito H, Takahashi R
PloS one 2011;6(5):e20427

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Guggulsterone targets smokeless tobacco induced PI3K/Akt pathway in head and neck cancer cells.

Macha MA, Matta A, Chauhan SS, Siu KW, Ralhan R
PloS one 2011 Feb 24;6(2):e14728

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Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex.

Drobic B, Pérez-Cadahía B, Yu J, Kung SK, Davie JR
Nucleic acids research 2010 Jun;38(10):3196-208

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Cyclin B1/Cdk1 phosphorylation of mitochondrial p53 induces anti-apoptotic response.

Nantajit D, Fan M, Duru N, Wen Y, Reed JC, Li JJ
PloS one 2010 Aug 23;5(8):e12341

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miR-451 protects against erythroid oxidant stress by repressing 14-3-3zeta.

Yu D, dos Santos CO, Zhao G, Jiang J, Amigo JD, Khandros E, Dore LC, Yao Y, D'Souza J, Zhang Z, Ghaffari S, Choi J, Friend S, Tong W, Orange JS, Paw BH, Weiss MJ
Genes & development 2010 Aug 1;24(15):1620-33

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Novel snail1 target proteins in human colon cancer identified by proteomic analysis.

Larriba MJ, Casado-Vela J, Pendás-Franco N, Peña R, García de Herreros A, Berciano MT, Lafarga M, Casal JI, Muñoz A
PloS one 2010 Apr 20;5(4):e10221

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Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase, and negatively charged membranes.

Halskau Ø Jr, Ying M, Baumann A, Kleppe R, Rodriguez-Larrea D, Almås B, Haavik J, Martinez A
The Journal of biological chemistry 2009 Nov 20;284(47):32758-69

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14-3-3zeta contributes to tyrosine hydroxylase activity in MN9D cells: localization of dopamine regulatory proteins to mitochondria.

Wang J, Lou H, Pedersen CJ, Smith AD, Perez RG
The Journal of biological chemistry 2009 May 22;284(21):14011-9

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Targeting of beta-arrestin2 to the centrosome and primary cilium: role in cell proliferation control.

Molla-Herman A, Boularan C, Ghossoub R, Scott MG, Burtey A, Zarka M, Saunier S, Concordet JP, Marullo S, Benmerah A
PloS one 2008;3(11):e3728

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Presence and distribution of 14-3-3 proteins in human ocular surface tissues.

Shankardas J, Senchyna M, Dimitrijevich SD
Molecular vision 2008;14:2604-15

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14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr.

Bolton DL, Barnitz RA, Sakai K, Lenardo MJ
Biology direct 2008 Apr 29;3:17

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Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein.

Park DJ, Freitas TA, Wallick CJ, Guyette CV, Warn-Cramer BJ
Protein science : a publication of the Protein Society 2006 Oct;15(10):2344-55

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14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase.

Pozuelo Rubio M, Peggie M, Wong BH, Morrice N, MacKintosh C
The EMBO journal 2003 Jul 15;22(14):3514-23

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HLS7, a hemopoietic lineage switch gene homologous to the leukemia-inducing gene MLF1

James H. Williams, Louise N. Daly, Evan Ingley, Jennifer G. Beaumont, Peta A. Tilbrook, Jean-Philippe Lalonde, Justin P. Stillitano, S. Peter Klinken
The EMBO Journal 1999 Oct;18(20):5559-5566

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 28

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in cells in tubules.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.