Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1882058 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) antibody
- Antibody type
- Monoclonal
- Antigen
- This YWHAZ antibody is generated from a mouse immunized with a recombinant protein from human YWHAZ.
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS. Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1314CT423-108-153-173-140
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
Submitted references Two unique 5' untranslated regions in mRNAs encoding human 14-3-3 zeta: differential expression in hemopoietic cells.
Cloning and expression of a human 14-3-3 protein mediating phospholipolysis. Identification of an arachidonoyl-enzyme intermediate during catalysis.
Seluja GA, Elias L, Pietromonaco SF
Biochimica et biophysica acta 1998 Feb 11;1395(3):281-7
Biochimica et biophysica acta 1998 Feb 11;1395(3):281-7
Cloning and expression of a human 14-3-3 protein mediating phospholipolysis. Identification of an arachidonoyl-enzyme intermediate during catalysis.
Zupan LA, Steffens DL, Berry CA, Landt M, Gross RW
The Journal of biological chemistry 1992 May 5;267(13):8707-10
The Journal of biological chemistry 1992 May 5;267(13):8707-10
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody (ABIN1882058) . AM2256a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.