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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4027 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4027, RRID:AB_1579838
- Product name
- YWHAZ polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of YWHAZ.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The interaction between ADAM 22 and 14-3-3zeta: regulation of cell adhesion and spreading.
Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.
Regulation of TSC2 by 14-3-3 binding.
Zhu Pc, Sun Y, Xu R, Sang Y, Zhao J, Liu G, Cai L, Li C, Zhao S
Biochemical and biophysical research communications 2003 Feb 21;301(4):991-9
Biochemical and biophysical research communications 2003 Feb 21;301(4):991-9
Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.
Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR
Molecular and cellular biology 2003 Aug;23(15):5376-87
Molecular and cellular biology 2003 Aug;23(15):5376-87
Regulation of TSC2 by 14-3-3 binding.
Li Y, Inoki K, Yeung R, Guan KL
The Journal of biological chemistry 2002 Nov 22;277(47):44593-6
The Journal of biological chemistry 2002 Nov 22;277(47):44593-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The YWHAZ polyclonal antibody (Cat # PAB4027) is used in Western blot to detect YWHAZ in Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with YWHAZ polyclonal antibody (Cat # PAB4027) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
