H00007534-M04

antibody from Abnova Corporation

Targeting: YWHAZ 14-3-3-zeta, KCIP-1, YWHAD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007534-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007534-M04, RRID:AB_10718671

Product name

YWHAZ monoclonal antibody (M04), clone 1B3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant YWHAZ.

Antigen sequence

VVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREK
IETELRDICNDVLSLLEKFLIPNASQAESKVFYLK
MKGDYYRYLAEVAAGDDKKGIVDQSQQAYQ


Isotype

IgG

Antibody clone number

1B3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of YWHAZ expression in transfected 293T cell line by YWHAZ monoclonal antibody (M04), clone 1B3.Lane 1: YWHAZ transfected lysate(27.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

YWHAZ monoclonal antibody (M04), clone 1B3. Western Blot analysis of YWHAZ expression in HL-60.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

YWHAZ monoclonal antibody (M04), clone 1B3. Western Blot analysis of YWHAZ expression in human kidney.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged YWHAZ is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol