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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- RQ5642 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- YWHAZ Antibody / 14-3-3 zeta/delta
- Antibody type
- Monoclonal
- Description
- This highly specific YWHAZ antibody is suitable for use in Western blot/Flow cytometry applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 6G5
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the YWHAZ antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human A549, 3) monkey COS-7, 4) human Raji, 5) human Caco-2, 6) human Jurkat, 7) mouse brain and 8) rat brain lysate with YWHAZ antibody. Predicted molecular weight ~28 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human PC-3 cells with YWHAZ antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAZ antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with YWHAZ antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAZ antibody.
