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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP21527PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21527PU-N, RRID:AB_10757420
- Product name
- anti 14-3-3 protein zeta/delta (200-245)
- Antibody type
- Polyclonal
- Antigen
- Amino acids 200-245 of human 14-3-3 zeta
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using AP21527PU-N at 2 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human spleen stained with 14-3-3 zeta antibody (Cat.-No.: AP21527PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
