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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- OAPC00209 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- 5-Lipoxygenase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100µl
- Storage
- For long term storage -20°C is recommended. Stable at -20°C for at least 1 year.
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Supportive validation
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- Aviva Systems Biology (provider)
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- Experimental details
- Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda -Ptase). The blot is identical to the control except that it was incubated in lambda -Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lambda -Ptase.
