10021-1-Ig

antibody from Proteintech Group

Targeting: ALOX5 5-LOX

Provider product page for 10021-1-Ig

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 10021-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10021-1-Ig, RRID:AB_513237
Product name: 5 Lipoxygenase antibody
Antibody type: Polyclonal
Description: KD/KO validated 5 Lipoxygenase antibody (Cat. #10021-1-Ig) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ALOX5 protein structure - 10021-1-Ig shown in red.

Submitted references ACSL1 Contributes to Metabolic-Associated Fatty Liver Disease by Mediating Arachidonic Acid Metabolism.

Tan M, Zhou Z, Peng C, Ke X, Long Q, Huang K
Lipids 2026 Mar;61(2):175-185

ALOX5 knockdown alleviates cartilage damage in osteoarthritis by inhibiting the NF-κB signaling pathway.

Cheng G, Chen K, Wang Z, Chen H, Shen Y
Cytokine 2026 Jun;202:157150

Sheng-Jiang Powder Alleviates Th17/Treg Immune Imbalance of Experimental Autoimmune Encephalomyelitis by Regulating the Fatty Acids Metabolism.

Wu L, Xu H, Xia H, Peng L, Qin L, Gong Q, Zhao M, Wu Z, Zhao Y, Zheng Z
Drug design, development and therapy 2025;19:4755-4776

Zileuton protects against arachidonic acid/5-lipoxygenase/leukotriene axis-mediated neuroinflammation in experimental traumatic brain injury.

Xu X, Wang Y, Li X, Yang Y, Yang D, Tang W, Lu J, Yuan F
Frontiers in pharmacology 2025;16:1516836

Lipid metabolism-related genes correlate with immune microenvironment and regulate the efficacy of immunotherapy via ferroptosis in melanoma.

Zhang H, Chen Y, Guo S, Guo W
Discover oncology 2025 Nov 25;16(1):2297

Verbascoside (VB) Inhibits Ischemic Stroke Progression via Suppression of Cell Damage and Ferroptosis By Targeting LCN2/ALOX5/SLC7A11/GPX4 Expression.

Zhang C, You J, Zhou X, Feng J, Li Q, Zhou H
Molecular neurobiology 2025 Nov 21;63(1):116

Whole-gene CRISPR/cas9 library screen revealed targeting STAT6 increased the sensitivity of liver cancer to celecoxib via inhibiting arachidonic acid shunting.

Hu C, Zeng Z, Bao X, Li D, Tai H, Zeng H, Luo C, Tang L, Chen T, Zuo S
Cell communication and signaling : CCS 2025 Aug 28;23(1):384

P2X7-CaMKII drives 5-LOX nuclear translocation to impair microglial function after subarachnoid hemorrhage.

Ding PF, Zhang JT, Zhu XL, Cui Y, Chen CL, Liu XZ, Shen JD, Zou LX, Lu Y, Zhuang Z, Hang CH, Li W
Journal of neuroinflammation 2025 Aug 28;22(1):208

Arachidonic acid enhances hepatocyte bile acid uptake and alleviates cholestatic liver disease by upregulating OATP1 expression.

Ma Y, Zou C, Yang Y, Fang M, Guan Y, Sun J, Gao Y, Shang Z, Zhang X
Food & function 2024 Sep 30;15(19):9916-9927

Central role of Sigma-1 receptor in ochratoxin A-induced ferroptosis.

Chen W, Han L, Yang R, Wang H, Yao S, Deng H, Liu S, Zhou Y, Shen XL
Archives of toxicology 2024 Oct;98(10):3323-3336

Loureirin B improves H/R-induced hepatic ischemia-reperfusion injury by downregulating ALOX5 to regulate mitochondrial homeostasis.

Yu Z, Xie S
Naunyn-Schmiedeberg's archives of pharmacology 2024 Oct;397(10):7561-7571

Inhibition of neutrophil swarming by type I interferon promotes intracellular bacterial evasion.

Li S, Yao Q, Li J, Yang H, Qian R, Zheng M, Wu N, Jiang H, Li L, Zeng Z
Nature communications 2024 Oct 7;15(1):8663

YTHDF1-regulated ALOX5 in retinal pigment epithelial cells under hypoxia enhances VEGF expression and promotes viability, migration, and angiogenesis of vascular endothelial cells.

Dong Y, Qian C, Yan P, Wan G
Scientific reports 2024 Oct 5;14(1):23226

Resolvin E1 and Inhibition of BLT2 Signaling Attenuate the Inflammatory Response and Improve One-Lung Ventilation-Induced Lung Injury.

Ji L, Liu G, Yu G, Xia C, Liu S, Lan Y
Immunological investigations 2024 Nov;53(8):1293-1307

ALOX5 acts as a key role in regulating the immune microenvironment in intrahepatic cholangiocarcinoma, recruiting tumor-associated macrophages through PI3K pathway.

Chen J, Tang Y, Qin D, Yu X, Tong H, Tang C, Tang Z
Journal of translational medicine 2023 Dec 20;21(1):923

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

Hu WM, Liu SQ, Zhu KF, Li W, Yang ZJ, Yang Q, Zhu ZC, Chang J
International immunopharmacology 2023 Aug;121:110505

Targeting Circulating lncRNA ENST00000538705.1 Relieves Acute Coronary Syndrome via Modulating ALOX15.

Chen H, Huang S, Guan F, Han S, Ye F, Li X, You L
Disease markers 2022;2022:8208471

Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p.

Zheng M, Fan P, Yang P, Zheng J, Zhao D
Journal of immunology research 2022;2022:4086710

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway.

Zhou T, Yan K, Zhang Y, Zhu L, Liao Y, Zheng X, Chen Y, Li X, Liu Z, Zhang Z
Frontiers in pharmacology 2022;13:1000254

Lipoxin A(4) and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Xu F, Zhang J, Zhou X, Hao H
Experimental cell research 2022 Mar 1;412(1):113025

Comprehensive Analysis of the Tumor Microenvironment and Ferroptosis-Related Genes Predict Prognosis with Ovarian Cancer.

Li XX, Xiong L, Wen Y, Zhang ZJ
Frontiers in genetics 2021;12:774400

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Xu Y, Denning KL, Lu Y
Biochemical pharmacology 2021 Oct;192:114678

MK591, a second generation leukotriene biosynthesis inhibitor, prevents invasion and induces apoptosis in the bone-invading C4-2B human prostate cancer cells: implications for the treatment of castration-resistant, bone-metastatic prostate cancer.

Sarveswaran S, Ghosh R, Morisetty S, Ghosh J
PloS one 2015;10(4):e0122805

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

Sarveswaran S, Chakraborty D, Chitale D, Sears R, Ghosh J
The Journal of biological chemistry 2015 Feb 20;290(8):4994-5006

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig( ALOX5 Antibody) at dilution of 1:600
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line