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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PPS001 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal 5-Lipoxygenase Antibody
- Antibody type
- Polyclonal
- Description
- 5-Lipoxygenase
- Reactivity
- Human, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Phospho-5-Lipoxygenase (S523) by Western Blot. Western blot of rat brain (cortex) tissue lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at S523 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-5-Lipoxygenase (S523). The immunolabeling is completely eliminated by treatment with lambda-PPase.
