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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AP08609PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP08609PU-N, RRID:AB_1975462
- Product name
- anti ALOX5 / LOG5 pSer523
- Antibody type
- Polyclonal
- Antigen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-Lipoxygenase (5-LO).
- Reactivity
- Human, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
Submitted references Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.
Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG
The Journal of biological chemistry 2004 Oct 1;279(40):41512-20
The Journal of biological chemistry 2004 Oct 1;279(40):41512-20
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
