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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA309073 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Anti-5-Lipoxygenase (Ser523) Antibody (Phospho-Specific)
- Antibody type
- Polyclonal
- Description
- Rabbit Anti-5-Lipoxygenase (Ser523) Antibody (Phospho-Specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ALOX5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: ??-Ptase). The blot is identical to the control except that it was incubated in ??-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with ??-Ptase.
- Validation comment
- WB
