PAB9606

antibody from Abnova Corporation

Targeting: ALOX5 5-LOX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9606

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9606, RRID:AB_1676186

Product name

ALOX5 (phospho S523) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of ALOX5.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.

Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG
The Journal of biological chemistry 2004 Oct 1;279(40):41512-20

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Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.

Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M
The New England journal of medicine 2004 Jan 1;350(1):29-37

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Prostaglandins and leukotrienes: advances in eicosanoid biology.

Funk CD
Science (New York, N.Y.) 2001 Nov 30;294(5548):1871-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of ALOX5 phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the ALOX5 (phospho S523) polyclonal antibody (Cat # PAB9606). The immunolabeling is completely eliminated by treatment with lambda-Ptase.