MA5-38050

antibody from Invitrogen Antibodies

Targeting: ALOX5 5-LOX

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38050

Provider

Invitrogen Antibodies

Product name

ALOX5 Recombinant Rabbit Monoclonal Antibody (5C5Z2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: Raji, Rat lung Immunogen sequence: CYRWITGDVE VVLRDGRAKL ARDDQIHILK QHRRKELETR QKQYRWMEWN PGFPLSIDAK CHKDLPRDIQ FDSEKGVDFV LNYSKAMENL FINRFMHMFQ S

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

5C5Z2

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Short-term high fat feeding induces inflammatory responses of tuft cells and mucosal barrier cells in the murine stomach.

Widmayer P, Pregitzer P, Breer H
Histology and histopathology 2023 Mar;38(3):273-286

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ALOX5 in extracts from Raji cells. Samples were incubated with ALOX5 Monoclonal antibody (Product # MA5-38050) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ALOX5 in extracts of Rat lung. Samples were incubated with ALOX5 Monoclonal antibody (Product # MA5-38050) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of ALOX5 in U-2 OS cells using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of ALOX5 in U-2 OS cells using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunocytochemistry) of ALOX5 in U-2 OS cells. Samples were incubated with ALOX5 Monoclonal antibody (Product # MA5-38050) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALOX5 in paraffin-embedded human appendix. Samples were incubated with ALOX5 Monoclonal antibody (Product # MA5-38050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALOX5 in paraffin-embedded mouse spleen. Samples were incubated with ALOX5 Monoclonal antibody (Product # MA5-38050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.