Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA1-46003 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALOX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: human Peripheral Blood Leukocyte protein.
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of LOX5 in human peripheral leukocytes using Product # PA1-46003 at 0.5 (Lane 1) and 2.0 µg/mL (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX5 in human peripheral leukocytes. Samples were incubated in ALOX5 polyclonal antibody (Product # PA1-46003). Lane 1: 0.5 µg/mL; Lane 2: 2.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX5 in 0.5 mg/mL Human Lung lysate. Samples were incubated in ALOX5 polyclonal antibody (Product # PA1-46003). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-ALOX5 Polyclonal Antibody (Product # PA1-46003) and a 70 kDa band corresponding to Arachidonate 5-lipoxygenase was observed in THP1 cells treated with both LPS and human TGF beta 1 and U2OS cells treated with Actinomycin D. Expression was also seen in Daudi, Raji and Raw 264.7 as compared to K-562, U-937, MOLT-4 and NIH/3T3 which are reported to be negative for ALOX5. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with Lipopolysaccharide (100 ng/mL) and human TGF beta 1 (1 ng/mL) for 72 hr (Lane 2), U-2 OS (Lane 3), U-2 OS treated with Actinomycin D (5nm for 24 hr) (Lane 4), Daudi (Lane 5), Raji (Lane 6), K-562 (Lane 7), U-937 (Lane 8), MOLT-4 (Lane 9), RAW 264.7 (Lane 10), NIH/3T3 (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) at ~55 kDa was also observed across the panel.