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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1644 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-5 Lipoxygenase/ALOX5 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for 5 LO/ALOX5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. 5 LO/ALOX5 information: Molecular Weight: 77983 MW; Subcellular Localization: Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-ALOX5 antibody, PA1644, Western blottingAll lanes: Anti ALOX5 (PA1644) at 0.5ug/ml Lane 1: SW620 Whole Cell Lysate at 40ug Lane 2: JURKAT Whole Cell Lysate at 40ug Lane 3: COLO320 Whole Cell Lysate at 40ug Lane 4: A549 Whole Cell Lysate at 40ug Lane 5: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 78KD Observed bind size: 78KD
- Additional image
