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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB4571 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL21/6Ckine Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant mouse CCL21/6Ckine in direct ELISAs and mouse CCL21/6Ckine in Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rhCCL3L1, rhCCL4L1, recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1g, 11, 12, 17, 19, 20, 22, 24, 25, 27, 28, rmEotaxin-3-like, recombinant rat (rr) CCL2, 20, rrEotaxin-3-like, recombinant canine CCL2, 3, 4, 5, recombinant cotton rat CCL2, 3, 4, 5, recombinant feline CCL5, or recombinant guinea pig CCL11 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P84444- Isotype
- IgG
- Antibody clone number
- 709420
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse CCL21/6Ckine by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB4571) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL21/6Ckine at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
