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Immunohistochemistry
Gel shiftAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA1-46110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AHR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- Suggested positive control: antigen standard for AHR (transient overexpression lysate). This antibody is not recommended for Western Blot applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Transcriptional and post-translational regulation of CYP1A1 by primaquine.
Werlinder V, Backlund M, Zhukov A, Ingelman-Sundberg M
The Journal of pharmacology and experimental therapeutics 2001 Apr;297(1):206-14
The Journal of pharmacology and experimental therapeutics 2001 Apr;297(1):206-14
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of AHR in formalin-fixed paraffin-embedded human liver. Samples were incubated in AHR polyclonal antibody (Product # PA1-46110) using a dilution of 1:200. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
