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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- GTX129012 - Provider product page
- Provider
- GeneTex
- Product name
- AHR antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AHR antibody detects AHR protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg whole cell lysate/extract of human AHR-transfected 293T cells (partial fragment)7.5 % SDS-PAGEAHR antibody (GTX129012) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with AHR antibody (GTX129012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and AHR knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with AHR antibody (GTX129012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AHR antibody detects AHR protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. AHR antibody (GTX129012) diluted at 1:500.
