Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-2289 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2289, RRID:AB_10002581
- Product name
- Rabbit Polyclonal AHR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Guinea Pig
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Pifithrin-α alters p53 post-translational modifications pattern and differentially inhibits p53 target genes.
Maternal Exposure to Bisphenol A Combined with High-Fat Diet-Induced Programmed Hypertension in Adult Male Rat Offspring: Effects of Resveratrol.
Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice.
Maternal Resveratrol Therapy Protects Male Rat Offspring against Programmed Hypertension Induced by TCDD and Dexamethasone Exposures: Is It Relevant to Aryl Hydrocarbon Receptor?
Exposure to diesel exhaust particulates induces cardiac dysfunction and remodeling.
Zhu J, Singh M, Selivanova G, Peuget S
Scientific reports 2020 Jan 23;10(1):1049
Scientific reports 2020 Jan 23;10(1):1049
Maternal Exposure to Bisphenol A Combined with High-Fat Diet-Induced Programmed Hypertension in Adult Male Rat Offspring: Effects of Resveratrol.
Hsu CN, Lin YJ, Tain YL
International journal of molecular sciences 2019 Sep 6;20(18)
International journal of molecular sciences 2019 Sep 6;20(18)
Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice.
Meng Y, Lin R, Wu F, Sun Q, Jia L
International journal of environmental research and public health 2018 Oct 1;15(10)
International journal of environmental research and public health 2018 Oct 1;15(10)
Maternal Resveratrol Therapy Protects Male Rat Offspring against Programmed Hypertension Induced by TCDD and Dexamethasone Exposures: Is It Relevant to Aryl Hydrocarbon Receptor?
Hsu CN, Lin YJ, Lu PC, Tain YL
International journal of molecular sciences 2018 Aug 20;19(8)
International journal of molecular sciences 2018 Aug 20;19(8)
Exposure to diesel exhaust particulates induces cardiac dysfunction and remodeling.
Bradley JM, Cryar KA, El Hajj MC, El Hajj EC, Gardner JD
Journal of applied physiology (Bethesda, Md. : 1985) 2013 Oct 1;115(7):1099-106
Journal of applied physiology (Bethesda, Md. : 1985) 2013 Oct 1;115(7):1099-106
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).