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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29642 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-AHR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-29642 targets AHR in IHC (P), IF and WB applications and shows reactivity with Human samples. The PA5-29642 immunogen is recombinant fragment corresponding to a region within amino acids 126 and 353 of Human AHR.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.77 mg/ml
- Storage
- -20°C
Submitted references Xeno-sensing activity of the aryl hydrocarbon receptor in human pluripotent stem cell-derived hepatocyte-like cells.
Kim HM, Kim JW, Choi Y, Chun HS, Im I, Han YM, Song CW, Yoon S, Park HJ
Scientific reports 2016 Feb 22;6:21684
Scientific reports 2016 Feb 22;6:21684
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AHR using 30µg of A) 293T and B) A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AHR polyclonal antibody (Product # PA5-29642) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AHR using 30 µg PC-3 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AHR polyclonal antibody (Product # PA5-29642) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AHR showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an AHR polyclonal antibody (Product # PA5-29642) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation and Western blot of AHR protein from 293T whole cell extracts using 5 µg of AHR antibody (Product # PA5-29642).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AHR in paraffin-embedded U87 xenograft using an AHR polyclonal antibody (Product # PA5-29642) at a dilution of 1:500.
