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antibody from Novus Biologicals
Targeting: AHR
bHLHe76
Western blot Immunocytochemistry
Immunohistochemistry Gel shift Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- NB100-128 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-128, RRID:AB_10001635
- Product name
- Goat Polyclonal AHR Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Vial size
- 1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Aromatic hydrocarbon receptor regulates chicken cytochrome P450 1A5 transcription: A novel insight into T-2 toxin-induced gene expression and cytotoxicity in LMH cells.
Cross-talk between aryl hydrocarbon receptor and the inflammatory response: a role for nuclear factor-κB.
Aryl hydrocarbon receptor signaling regulates NF-κB RelB activation during dendritic-cell differentiation.
Interaction of aryl hydrocarbon receptor and NF-κB subunit RelB in breast cancer is associated with interleukin-8 overexpression.
Aryl hydrocarbon receptor is activated by glucose and regulates the thrombospondin-1 gene promoter in endothelial cells.
Combined screening of thymocytes using apoptosis-specific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity.
A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.
Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene.
Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene.
Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL.
Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL.
Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements.
Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements.
Baculovirus expression of the Ah receptor and Ah receptor nuclear translocater. Evidence for additional dioxin responsive element-binding species and factors required for signaling.
Characterization of polyclonal antibodies to the Ah receptor prepared by immunization with a synthetic peptide hapten.
Liu Q, Wen J, Zhu J, Zhang T, Deng Y, Jiang J
Biochemical pharmacology 2019 Oct;168:319-329
Biochemical pharmacology 2019 Oct;168:319-329
Cross-talk between aryl hydrocarbon receptor and the inflammatory response: a role for nuclear factor-κB.
Vogel CF, Khan EM, Leung PS, Gershwin ME, Chang WL, Wu D, Haarmann-Stemmann T, Hoffmann A, Denison MS
The Journal of biological chemistry 2014 Jan 17;289(3):1866-75
The Journal of biological chemistry 2014 Jan 17;289(3):1866-75
Aryl hydrocarbon receptor signaling regulates NF-κB RelB activation during dendritic-cell differentiation.
Vogel CF, Wu D, Goth SR, Baek J, Lollies A, Domhardt R, Grindel A, Pessah IN
Immunology and cell biology 2013 Oct;91(9):568-75
Immunology and cell biology 2013 Oct;91(9):568-75
Interaction of aryl hydrocarbon receptor and NF-κB subunit RelB in breast cancer is associated with interleukin-8 overexpression.
Vogel CF, Li W, Wu D, Miller JK, Sweeney C, Lazennec G, Fujisawa Y, Matsumura F
Archives of biochemistry and biophysics 2011 Aug 1;512(1):78-86
Archives of biochemistry and biophysics 2011 Aug 1;512(1):78-86
Aryl hydrocarbon receptor is activated by glucose and regulates the thrombospondin-1 gene promoter in endothelial cells.
Dabir P, Marinic TE, Krukovets I, Stenina OI
Circulation research 2008 Jun 20;102(12):1558-65
Circulation research 2008 Jun 20;102(12):1558-65
Combined screening of thymocytes using apoptosis-specific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity.
Fisher MT, Nagarkatti M, Nagarkatti PS
Toxicological sciences : an official journal of the Society of Toxicology 2004 Mar;78(1):116-24
Toxicological sciences : an official journal of the Society of Toxicology 2004 Mar;78(1):116-24
A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.
Fukuda I, Nishiumi S, Yabushita Y, Mukai R, Kodoi R, Hashizume K, Mizuno M, Hatanaka Y, Ashida H
Journal of immunological methods 2004 Apr;287(1-2):187-201
Journal of immunological methods 2004 Apr;287(1-2):187-201
Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene.
Tsuchiya Y, Nakajima M, Yokoi T
Journal of biochemistry 2003 May;133(5):583-92
Journal of biochemistry 2003 May;133(5):583-92
Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene.
Tsuchiya Y, Nakajima M, Yokoi T
Journal of biochemistry 2003 May;133(5):583-92
Journal of biochemistry 2003 May;133(5):583-92
Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL.
Hayashibara T, Yamada Y, Mori N, Harasawa H, Sugahara K, Miyanishi T, Kamihira S, Tomonaga M
Biochemical and biophysical research communications 2003 Jan 3;300(1):128-34
Biochemical and biophysical research communications 2003 Jan 3;300(1):128-34
Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL.
Hayashibara T, Yamada Y, Mori N, Harasawa H, Sugahara K, Miyanishi T, Kamihira S, Tomonaga M
Biochemical and biophysical research communications 2003 Jan 3;300(1):128-34
Biochemical and biophysical research communications 2003 Jan 3;300(1):128-34
Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements.
Minsavage GD, Vorojeikina DP, Gasiewicz TA
Archives of biochemistry and biophysics 2003 Apr 1;412(1):95-105
Archives of biochemistry and biophysics 2003 Apr 1;412(1):95-105
Mutational analysis of the mouse aryl hydrocarbon receptor tyrosine residues necessary for recognition of dioxin response elements.
Minsavage GD, Vorojeikina DP, Gasiewicz TA
Archives of biochemistry and biophysics 2003 Apr 1;412(1):95-105
Archives of biochemistry and biophysics 2003 Apr 1;412(1):95-105
Baculovirus expression of the Ah receptor and Ah receptor nuclear translocater. Evidence for additional dioxin responsive element-binding species and factors required for signaling.
Chan WK, Chu R, Jain S, Reddy JK, Bradfield CA
The Journal of biological chemistry 1994 Oct 21;269(42):26464-71
The Journal of biological chemistry 1994 Oct 21;269(42):26464-71
Characterization of polyclonal antibodies to the Ah receptor prepared by immunization with a synthetic peptide hapten.
Poland A, Glover E, Bradfield CA
Molecular pharmacology 1991 Jan;39(1):20-6
Molecular pharmacology 1991 Jan;39(1):20-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 20 ug/ml conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.
