Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AF6697 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse AHR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse AHR in Western blots.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P30561
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse AHR by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse AHR by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, Hepa 1-6 mouse hepatoma cell line, Neuro-2A mouse neuroblastoma cell line (negative control), NIH-3T3 mouse embryonic fibroblast cell line (negative control), and mouse cerebellum tissue (negative control). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- AHR in C2C12 Mouse Cell Line. AHR was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.