AP6533b

antibody from Abcepta

Targeting: ACTR2 ARP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6533b

Provider

Abcepta

Proper citation

Abgent Cat#AP6533b, RRID:AB_1967172

Product name

ACTR2 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of ACTR2 Antibody (C-term) (Cat. #AP6533b) in MCF7,A2058 cell line lysates(35ug/lane). ACTR2 (arrow) was detected using the purified Pab."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

ACTR2 Antibody (C-term) (Cat. #AP6533b) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50