Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA5-61401 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arp3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VLSGGSTMFR DFGRRLQRDL KRTVDARLKL SEELSGGR Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Arp3 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an Arp3 Polyclonal Antibody (Product # PA5-61401).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Arp3 was achieved by transfecting MCF-7 cells with Arp3 specific siRNAs (Silencer® select Product # s19640, s19641). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF-7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Arp3 Rabbit Polyclonal Antibody (Product # PA5-61401, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Arp3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-61401) and a ~47 kDa band was observed across the panel tested. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), PC-3 (Lane 2), U-87 MG (Lane 3), HeLa (Lane 4), U-2 OS (Lane 5), MCF-7 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-61401) and a ~47 kDa band was observed across the tissue panel tested except in Mouse and Rat Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Thymus (Lane 1), Mouse Spleen (Lane 2), Mouse Testis (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Liver (Lane 5), Rat Thymus (Lane 6), Rat Spleen (Lane 7), Rat Skeletal Muscle (Lane 8) and Rat Liver (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Arp3 in human cerebral cortex tissue shows moderate nuclear positivity in neuronal cells. Samples were probed using an Arp3 Polyclonal Antibody (Product # PA5-61401).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Arp3 in human cerebral cortex tissue shows moderate nuclear positivity in neuronal cells. Samples were probed using an Arp3 Polyclonal Antibody (Product # PA5-61401).