Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-62459 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arp3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KPTYATKWPI RHGIVEDWDL MERFMEQVIF KYLRAEPEDH Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Arp3 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an Arp3 Polyclonal Antibody (Product # PA5-62459).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Arp3 was achieved by transfecting MCF-7 cells with Arp3 specific siRNAs (Silencer® select Product # s19640, s19641). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF-7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Arp3 Rabbit Polyclonal Antibody (Product # PA5-62459, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Arp3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-62459) and a ~47 kDa band was observed across the panel tested. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), PC-3 (Lane 2), U-87 MG (Lane 3), HeLa (Lane 4), U-2 OS (Lane 5) and MCF-7 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-62459) and a ~47 kDa band was observed across the tissue panel tested except Mouse and Rat Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Thymus (Lane 1), Mouse Spleen (Lane 2), Mouse Skeletal Muscle (Lane 3), Mouse Liver (Lane 4), Rat Thymus (Lane 5), Rat Spleen (Lane 6) and Rat Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Arp3 in human stomach tissue., upper shows strong cytoplasmic positivity in glandular cells. Samples were probed using an Arp3 Polyclonal Antibody (Product # PA5-62459).