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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab089664 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Arp3 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Arp3 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Applicationá WB: 1/500-1/1000 á IHC: 1/50-1/100 á IF/ICC: 1/50-1/200 Protein RelevanceThe Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 394-407- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,3 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Arp3 Antibody (Ab089664) - Western Blot All lanes: Recombinant Arp3 Antibody (Ab089664) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 28.2 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Arp3 Antibody (Ab089664) - IF/ICC Immunocytochemistry analysis of Arp3 (green) in HeLa cells using Recombinant Arp3 Antibody (Ab089664), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Arp3 Antibody (Ab089664) - IHC Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Arp3 Antibody (Ab089664). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
