H00000640-M01

antibody from Abnova Corporation

Targeting: BLK MGC10442

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000640-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000640-M01, RRID:AB_425327

Product name

BLK monoclonal antibody (M01), clone 3E5-3A8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant BLK.

Antigen sequence

MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDA
PPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYT
AMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGRE
GYVPSNFVARVESLEMERWFFRSQGRKEAERQLLA
PINKAGSFLIRESETNKGAFSLSVKDVTTQGELIK
HYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGD
GLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVR
KLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEA
FLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYM
ARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAY
IERMNSIHRDLRAANILVSEALCCKIADFGLARII
DSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWS
FGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMP
RPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVL
EDFYTATERQYELQP


Isotype

IgG

Antibody clone number

3E5-3A8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M01), clone 3E5-3A8.Lane 1: BLK transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BLK monoclonal antibody (M01), clone 3E5-3A8. Western Blot analysis of BLK expression in A-431.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BLK is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol