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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3417 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3417, RRID:AB_1571720
- Product name
- BLK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BLK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cloning and chromosomal localization of the human homologue of a B-lymphocyte specific protein tyrosine kinase (blk).
Molecular cloning, characterization, and chromosomal localization of a human lymphoid tyrosine kinase related to murine Blk.
Drebin JA, Hartzell SW, Griffin C, Campbell MJ, Niederhuber JE
Oncogene 1995 Feb 2;10(3):477-86
Oncogene 1995 Feb 2;10(3):477-86
Molecular cloning, characterization, and chromosomal localization of a human lymphoid tyrosine kinase related to murine Blk.
Islam KB, Rabbani H, Larsson C, Sanders R, Smith CI
Journal of immunology (Baltimore, Md. : 1950) 1995 Feb 1;154(3):1265-72
Journal of immunology (Baltimore, Md. : 1950) 1995 Feb 1;154(3):1265-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BLK polyclonal antibody (Cat # PAB3417) in mouse kidney tissue lysate. BLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BLK polyclonal antibody (Cat # PAB3417) transiently transfected HEK293 cell line lysate (1 ug/lane). BLK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BLK polyclonal antibody (Cat # PAB3417) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
