MA5-15725

antibody from Invitrogen Antibodies

Targeting: BLK MGC10442

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15725

Provider

Invitrogen Antibodies

Product name

BLK Monoclonal Antibody (1E6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15725 targets BLK in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human samples. The MA5-15725 immunogen is purified recombinant fragment of human BLK expressed in E. Coli. MA5-15725 detects BLK which has a predicted molecular weight of approximately 58kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1E6

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BLK using BLK monoclonal antibody (Product # MA5-15725) in HEK293 (1) and BLK (AA: 2-200) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BLK using BLK monoclonal antibody (Product # MA5-15725) in HEK293 (1) and BLK (AA: 2-200) human IgG Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using BLK monoclonal antibody (Product # MA5-15725) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using BLK monoclonal antibody (Product # MA5-15725) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HL-60 cells using BLK monoclonal antibody (Product # MA5-15725) (green) and negative control (purple).