LS-C149890

antibody from LSBio

Targeting: CA9 CAIX, MN

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C149890

Provider

LSBio

Product name

Anti-CA9 / Carbonic Anhydrase IX Antibody (aa59-414) LS-C149890

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

25µg

Storage

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Carbonic Anhydrase IX/CA9 by Western Blot. Western blot shows lysates of U-87 MG human glioblastoma/ astrocytoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carbonic Anhydrase IX/CA9 at approximately 58 kD (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Carbonic Anhydrase IX/CA9 in Human Colon Cancer Tissue. Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells.