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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- GTX629747 - Provider product page
- Provider
- GeneTex
- Product name
- Carbonic Anhydrase IX antibody [GT14212]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract (untreated)B. 30 ?g HeLa whole cell lysate/extract (200 ?M CoCl2 treatment for 24 hr) C. 30u g HeLa whole cell lysate/extract (200 ?M CoCl2 treatment for 48 hr)D. 30 ?g HeLa whole cell lysate/extract (untreated)E. 30 ?g HeLa whole cell lysate/extract (1% O2 treatment for 24 hr) F. 30 ?g HeLa whole cell lysate/extract (1% O2 treatment for 48 hr)7.5 % SDS-PAGECarbonic Anhydrase IX antibody [GT14212] (GTX629747) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis.Sample: 200£gM CoCl2 for 48 hr A431 cells were fixed in ice-cold MeOH for 5 min.Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody [GT14212] (GTX629747) diluted at 1:100.Blue: Hoechst 33342 staining.
