TA500623

antibody from OriGene

Targeting: CA9 CAIX, MN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500623

Provider

OriGene

Proper citation

OriGene Cat#TA500623, RRID:AB_2259265

Product name

Anti-CA9 (Carbonic Anhydrase IX) mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)

Antibody type

Monoclonal

Description

Anti-CA9 (Carbonic Anhydrase IX) mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)

Host

Mouse

Conjugate

Unconjugated

Epitope

CA9

Isotype

IgG

Antibody clone number

OTI1G7

Vial size

100 µl

Concentration

1 mg/ml

References

Submitted references

CREB is a key negative regulator of carbonic anhydrase IX (CA9) in gastric cancer.

Wang G, Cheng Z, Liu F, Zhang H, Li J, Li F
Cellular signalling 2015 Jul;27(7):1369-79

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA9 (RC204839, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA9.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-CA9mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500623, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY CA9(RC204839)(Red) or empty vector control plasmid(Blue) were immunostained with anti-CA9 mouse monoclonal(TA500623, Dilution 1:1,000), and then analyzed by flow cytometry.

Validation comment

FC