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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 66243-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CA9 Monoclonal Antibody (1G4G5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G4G5
- Vial size
- 150 µL
- Concentration
- 1.36 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig stomach tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig brain tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human stomach tissue were subjected to SDS PAGE followed by western blot with 66243-1-IG ( CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEK-293 cells using 66243-1-IG (CA9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-IG ( CA9 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-IG ( CA9 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HEK-293 cells were stained with 0.20 µg/test Anti-Human CA9 (Product # 66243-1-IG, Clone:1G4G5) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1,000. Fixed with90% MeOH.
