V8462

antibody from NSJ Bioreagents

Targeting: CA9 CAIX, MN

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8462

Provider

NSJ Bioreagents

Product name

CA9 Antibody / Carbonic Anhydrase IX

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

CA9/3405

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the Carbonic Anhydrase IX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human renal cell carcinoma with Carbonic Anhydrase IX antibody (clone CA9/3405). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of FPA fixed human U-87 MG cells with Carbonic Anhydrase IX antibody (clone CA9/3405); Red=isotype control, Blue= Carbonic Anhydrase IX antibody.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Carbonic Anhydrase IX antibody (clone CA9/3405). These results demonstrate the foremost specificity of the CA9/3405 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Carbonic Anhydrase IX antibody (clone CA9/3405) as confirmation of integrity and purity.