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- Antibody Data
- Antigen structure
- References [4]
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- ELISA [1]
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- Product number
- 500-P252-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTGF Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGF. Anti-Human CTGF-specific antibody was purified by affinity chromatography employing an immobilized Human CTGF matrix. Sandwich ELISA: To detect Human CTGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGF (500-P252Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGF. Western Blot: To detect Human CTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1.
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
P38 pathway as a key downstream signal of connective tissue growth factor to regulate metastatic potential in non-small-cell lung cancer.
CCN2 inhibits lung cancer metastasis through promoting DAPK-dependent anoikis and inducing EGFR degradation.
Sogi C, Takeshita N, Jiang W, Kim S, Maeda T, Yoshida M, Oyanagi T, Ito A, Kimura S, Seki D, Takano I, Sakai Y, Fujiwara I, Kure S, Takano-Yamamoto T
JBMR plus 2020 Jul;4(7):e10369
JBMR plus 2020 Jul;4(7):e10369
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
Yang SY, Lin SL, Chen YM, Wu VC, Yang WS, Wu KD
Scientific reports 2016 Sep 23;6:33705
Scientific reports 2016 Sep 23;6:33705
P38 pathway as a key downstream signal of connective tissue growth factor to regulate metastatic potential in non-small-cell lung cancer.
Kato S, Yokoyama S, Hayakawa Y, Li L, Iwakami Y, Sakurai H, Saiki I
Cancer science 2016 Oct;107(10):1416-1421
Cancer science 2016 Oct;107(10):1416-1421
CCN2 inhibits lung cancer metastasis through promoting DAPK-dependent anoikis and inducing EGFR degradation.
Chang CC, Yang MH, Lin BR, Chen ST, Pan SH, Hsiao M, Lai TC, Lin SK, Jeng YM, Chu CY, Chen RH, Yang PC, Chin YE, Kuo ML
Cell death and differentiation 2013 Mar;20(3):443-55
Cell death and differentiation 2013 Mar;20(3):443-55
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human CTGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CTGF Polyclonal Antibody (Product # 500-P252-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CTGF Polyclonal Antibody, Biotin (Product # 500-P252BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGF.
