- PA5-32193 - Invitrogen Antibodies CCN2 antibody

Submitted references Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.

Li H, Kan B, Song L, Liu Y, Jian X
The Journal of international medical research 2020 Sep;48(9):300060520905425

The mevalonate coordinates energy input and cell proliferation.

Gong L, Xiao Y, Xia F, Wu P, Zhao T, Xie S, Wang R, Wen Q, Zhou W, Xu H, Zhu L, Zheng Z, Yang T, Chen Z, Duan Q
Cell death & disease 2019 Apr 11;10(4):327

Synergistic Effect of Simvastatin Plus Radiation in Gastric Cancer and Colorectal Cancer: Implications of BIRC5 and Connective Tissue Growth Factor.

Lim T, Lee I, Kim J, Kang WK
International journal of radiation oncology, biology, physics 2015 Oct 1;93(2):316-25

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF using 50 µg of mouse heart lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CTGF polyclonal antibody (Product # PA5-32193) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a CTGF polyclonal antibody (Product # PA5-32193) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF using 30 µg of HUVEC lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CTGF polyclonal antibody (Product # PA5-32193) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF using 293T mock transfected (-) or transfected with a construct expressing human CTGF (+). Samples were loaded onto a 10% SDS-PAGE gel and probed with a CTGF polyclonal antibody (Product # PA5-32193) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF using 1 µg of rat bladder lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CTGF polyclonal antibody (Product # PA5-32193) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CTGF Polyclonal Antibody (Product # PA5-32193) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CTGF Polyclonal Antibody (Product # PA5-32193). HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF Polyclonal Antibody (Product # PA5-32193) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CTGF Polyclonal Antibody (Product # PA5-32193). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF Polyclonal Antibody (Product # PA5-32193) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CTGF was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CTGF Polyclonal Antibody (Product # PA5-32193) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTGF was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CTGF Polyclonal Antibody (Product # PA5-32193) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-CTGF Polyclonal Antibody (Product # PA5-32193) and a 38 kDa band corresponding to Connective tissue growth factor was observed across in HUVEC upon PTI treatment, but not in HEK 293 upon PTI treatment. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HUVEC (Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours) (Lane 2), HEK-293 (Lane 3), HEK-293 (Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours) (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CTGF was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CTGF Polyclonal Antibody (Product # PA5-32193) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel (a-d) shows representative HUVEC control cell, whereas Panel (e-h) represent HUVEC cells treated with Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours. Similarly, panel (i-l) represent HEK 293 control cells and Panel (m-p) represent HEK 293 treated with Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CTGF (Product # PA5-32193) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. Immunoblotting analysis of MST, TGF-beta1, Smad3, Yap and CTGF among groups over time. Actin was used as an internal reference. a. Protein expression levels of MST, TGF-beta1, Smad3, Yap, CTGF and actin. b. Quantification of MST protein expression in lung tissue. c. Quantification of Smad3 protein expression in lung tissue. d. Quantification of TGF-beta1 protein expression in lung tissue. e. Quantification of Yap protein expression in lung tissue. f. Quantification of CTGF protein expression in lung tissue. Exp vs BC: aa (P < 0.01), a (P < 0.05); Exp vs SY Int: bb (P < 0.01), b (P < 0.05); Exp vs SB Int: **(P < 0.01), *(P < 0.05).Abbreviations: BC, blank control; SY Con, safflower yellow pigment control; SB Con, SB431542 control; Exp, exposure; SY Int, safflower yellow pigment intervention; SB Int, SB431542 intervention; MST, mammalian STE20-like; TGF-beta1, transforming growth factor-beta1; CTGF, connective tissue growth factor.

PA5-32193

Antibody data