Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-21569 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Cytochrome c Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human Ex-Vivo Liver Model for Acetaminophen-induced Liver Damage.
Schreiter T, Sowa JP, Schlattjan M, Treckmann J, Paul A, Strucksberg KH, Baba HA, Odenthal M, Gieseler RK, Gerken G, Arteel GE, Canbay A
Scientific reports 2016 Aug 23;6:31916
Scientific reports 2016 Aug 23;6:31916
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Paraffin-embedded human colon cancer. Cytochrome C antibodydiluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.