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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8385 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8385, RRID:AB_2090472
- Product name
- Anti-CYCS
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
Submitted references Mitochondrial dysfunction links ceramide activated HRK expression and cell death.
Hepatocyte nuclear factor-4alpha is a central transactivator of the mouse Ntcp gene.
Comparison of herpes simplex virus reactivation in ganglia in vivo and in explants demonstrates quantitative and qualitative differences.
Rizvi F, Heimann T, Herrnreiter A, O'Brien WJ
PloS one 2011 Mar 31;6(3):e18137
PloS one 2011 Mar 31;6(3):e18137
Hepatocyte nuclear factor-4alpha is a central transactivator of the mouse Ntcp gene.
Geier A, Martin IV, Dietrich CG, Balasubramaniyan N, Strauch S, Suchy FJ, Gartung C, Trautwein C, Ananthanarayanan M
American journal of physiology. Gastrointestinal and liver physiology 2008 Aug;295(2):G226-33
American journal of physiology. Gastrointestinal and liver physiology 2008 Aug;295(2):G226-33
Comparison of herpes simplex virus reactivation in ganglia in vivo and in explants demonstrates quantitative and qualitative differences.
Sawtell NM, Thompson RL
Journal of virology 2004 Jul;78(14):7784-94
Journal of virology 2004 Jul;78(14):7784-94
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:2000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 12
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
