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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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- Product number
- AM20329PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Cytochrome c
- Antibody type
- Monoclonal
- Antigen
- Rat Cytochrome c-OVA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H2.B4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
Submitted references Adenovirus type 5 rupture of lysosomes leads to cathepsin B-dependent mitochondrial stress and production of reactive oxygen species.
Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease.
Alpha-fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation.
Early mitochondrial activation and cytochrome c up-regulation during apoptosis.
Abrin triggers cell death by inactivating a thiol-specific antioxidant protein.
A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells.
Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c.
McGuire KA, Barlan AU, Griffin TM, Wiethoff CM
Journal of virology 2011 Oct;85(20):10806-13
Journal of virology 2011 Oct;85(20):10806-13
Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease.
She P, Zhang Z, Marchionini D, Diaz WC, Jetton TJ, Kimball SR, Vary TC, Lang CH, Lynch CJ
American journal of physiology. Endocrinology and metabolism 2011 Jul;301(1):E49-61
American journal of physiology. Endocrinology and metabolism 2011 Jul;301(1):E49-61
Alpha-fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation.
Semenkova L, Dudich E, Dudich I, Tokhtamisheva N, Tatulov E, Okruzhnov Y, Garcia-Foncillas J, Palop-Cubillo JA, Korpela T
European journal of biochemistry / FEBS 2003 Nov;270(21):4388-99
European journal of biochemistry / FEBS 2003 Nov;270(21):4388-99
Early mitochondrial activation and cytochrome c up-regulation during apoptosis.
Chandra D, Liu JW, Tang DG
The Journal of biological chemistry 2002 Dec 27;277(52):50842-54
The Journal of biological chemistry 2002 Dec 27;277(52):50842-54
Abrin triggers cell death by inactivating a thiol-specific antioxidant protein.
Shih SF, Wu YH, Hung CH, Yang HY, Lin JY
The Journal of biological chemistry 2001 Jun 15;276(24):21870-7
The Journal of biological chemistry 2001 Jun 15;276(24):21870-7
A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells.
Jemmerson R, Johnson JG, Burrell E, Taylor PS, Jenkins MK
European journal of immunology 1991 Jan;21(1):143-51
European journal of immunology 1991 Jan;21(1):143-51
Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c.
Goshorn SC, Retzel E, Jemmerson R
The Journal of biological chemistry 1991 Feb 5;266(4):2134-42
The Journal of biological chemistry 1991 Feb 5;266(4):2134-42
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 µg 6H2.B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-Cytochrome c antibody (Cat.-No SM7019P). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In addition to the specific 15 kD Cytochrome c band immunoprecipitated by 6H2.B4, heavy and light immunoglobulin chains are recognized by the Goat anti-Mouse secondary antibody.
