Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [10]
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Validation data
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- Product number
- 33-8500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome C Monoclonal Antibody (7H8.2C12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Drosophila, Rabbit, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7H8.2C12
- Vial size
- 200 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Effect of immune modulation on the skeletal muscle mitochondrial exercise response: An exploratory study in mice with cancer.
Impact of Exercise and Detraining during Childhood on Brown Adipose Tissue Whitening in Obesity.
The Effects of Potassium Cyanide on the Functional Recovery of Isolated Rat Hearts after Ischemia and Reperfusion: The Role of Oxidative Stress.
Tumor protein Tctp regulates axon development in the embryonic visual system.
Morphine stimulates nitric oxide release in human mitochondria.
The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.
Targeting the GD3 acetylation pathway selectively induces apoptosis in glioblastoma.
Protective effect of sulforaphane against cisplatin-induced mitochondrial alterations and impairment in the activity of NAD(P)H: quinone oxidoreductase 1 and γ glutamyl cysteine ligase: studies in mitochondria isolated from rat kidney and in LLC-PK1 cells.
Lindane and cell death: at the crossroads between apoptosis, necrosis and autophagy.
Mitochondrial disruption in Drosophila apoptosis.
Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation.
Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.
Cadmium-induced apoptosis through the mitochondrial pathway in rainbow trout hepatocytes: involvement of oxidative stress.
Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.
Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.
Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.
Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation.
Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines.
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
Buss LA, Hock B, Merry TL, Ang AD, Robinson BA, Currie MJ, Dachs GU
PloS one 2021;16(10):e0258831
PloS one 2021;16(10):e0258831
Impact of Exercise and Detraining during Childhood on Brown Adipose Tissue Whitening in Obesity.
Takaishi K, Oshima T, Eto H, Nishihira M, Nguyen ST, Ochi R, Fujita N, Urakawa S
Metabolites 2021 Oct 1;11(10)
Metabolites 2021 Oct 1;11(10)
The Effects of Potassium Cyanide on the Functional Recovery of Isolated Rat Hearts after Ischemia and Reperfusion: The Role of Oxidative Stress.
Petkovic AM, Jakovljevic VL, Bradic JV, Jeremic JN, Jeremic NS, Nikolic Turnic TR, Jovicic NU, Rosic VZ, Srejovic IM, Zivkovic VI
Oxidative medicine and cellular longevity 2018;2018:5979721
Oxidative medicine and cellular longevity 2018;2018:5979721
Tumor protein Tctp regulates axon development in the embryonic visual system.
Roque CG, Wong HH, Lin JQ, Holt CE
Development (Cambridge, England) 2016 Apr 1;143(7):1134-48
Development (Cambridge, England) 2016 Apr 1;143(7):1134-48
Morphine stimulates nitric oxide release in human mitochondria.
Stefano GB, Mantione KJ, Capellan L, Casares FM, Challenger S, Ramin R, Samuel JM, Snyder C, Kream RM
Journal of bioenergetics and biomembranes 2015 Oct;47(5):409-17
Journal of bioenergetics and biomembranes 2015 Oct;47(5):409-17
The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.
Hedgepeth SC, Garcia MI, Wagner LE 2nd, Rodriguez AM, Chintapalli SV, Snyder RR, Hankins GD, Henderson BR, Brodie KM, Yule DI, van Rossum DB, Boehning D
The Journal of biological chemistry 2015 Mar 13;290(11):7304-13
The Journal of biological chemistry 2015 Mar 13;290(11):7304-13
Targeting the GD3 acetylation pathway selectively induces apoptosis in glioblastoma.
Birks SM, Danquah JO, King L, Vlasak R, Gorecki DC, Pilkington GJ
Neuro-oncology 2011 Sep;13(9):950-60
Neuro-oncology 2011 Sep;13(9):950-60
Protective effect of sulforaphane against cisplatin-induced mitochondrial alterations and impairment in the activity of NAD(P)H: quinone oxidoreductase 1 and γ glutamyl cysteine ligase: studies in mitochondria isolated from rat kidney and in LLC-PK1 cells.
Guerrero-Beltrán CE, Calderón-Oliver M, Martínez-Abundis E, Tapia E, Zarco-Márquez G, Zazueta C, Pedraza-Chaverri J
Toxicology letters 2010 Nov 10;199(1):80-92
Toxicology letters 2010 Nov 10;199(1):80-92
Lindane and cell death: at the crossroads between apoptosis, necrosis and autophagy.
Zucchini-Pascal N, de Sousa G, Rahmani R
Toxicology 2009 Feb 4;256(1-2):32-41
Toxicology 2009 Feb 4;256(1-2):32-41
Mitochondrial disruption in Drosophila apoptosis.
Abdelwahid E, Yokokura T, Krieser RJ, Balasundaram S, Fowle WH, White K
Developmental cell 2007 May;12(5):793-806
Developmental cell 2007 May;12(5):793-806
Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation.
Tanaka S, Takehashi M, Iida S, Kitajima T, Kamanaka Y, Stedeford T, Banasik M, Ueda K
Journal of neurochemistry 2005 Oct;95(1):179-90
Journal of neurochemistry 2005 Oct;95(1):179-90
Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.
Reiss U, Oskouian B, Zhou J, Gupta V, Sooriyakumaran P, Kelly S, Wang E, Merrill AH Jr, Saba JD
The Journal of biological chemistry 2004 Jan 9;279(2):1281-90
The Journal of biological chemistry 2004 Jan 9;279(2):1281-90
Cadmium-induced apoptosis through the mitochondrial pathway in rainbow trout hepatocytes: involvement of oxidative stress.
Risso-de Faverney C, Orsini N, de Sousa G, Rahmani R
Aquatic toxicology (Amsterdam, Netherlands) 2004 Aug 25;69(3):247-58
Aquatic toxicology (Amsterdam, Netherlands) 2004 Aug 25;69(3):247-58
Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.
Wilkinson BL, Elam JS, Fadool DA, Hyson RL
Neuroscience 2003;120(4):1071-9
Neuroscience 2003;120(4):1071-9
Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.
Abarca-Rojano E, Rosas-Medina P, Zamudio-Cortéz P, Mondragón-Flores R, Sánchez-García FJ
Scandinavian journal of immunology 2003 Oct;58(4):419-27
Scandinavian journal of immunology 2003 Oct;58(4):419-27
Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.
Abarca-Rojano E, Rosas-Medina P, Zamudio-Cortéz P, Mondragón-Flores R, Sánchez-García FJ
Scandinavian journal of immunology 2003 Oct;58(4):419-27
Scandinavian journal of immunology 2003 Oct;58(4):419-27
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.
Love DC, Kochan J, Cathey RL, Shin SH, Hanover JA
Journal of cell science 2003 Feb 15;116(Pt 4):647-54
Journal of cell science 2003 Feb 15;116(Pt 4):647-54
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.
Love DC, Kochan J, Cathey RL, Shin SH, Hanover JA
Journal of cell science 2003 Feb 15;116(Pt 4):647-54
Journal of cell science 2003 Feb 15;116(Pt 4):647-54
Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation.
Yu F, Finley RL Jr, Raz A, Kim HR
The Journal of biological chemistry 2002 May 3;277(18):15819-27
The Journal of biological chemistry 2002 May 3;277(18):15819-27
Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines.
Hetz C, Bono MR, Barros LF, Lagos R
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 5;99(5):2696-701
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 5;99(5):2696-701
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.
Olichon A, Emorine LJ, Descoins E, Pelloquin L, Brichese L, Gas N, Guillou E, Delettre C, Valette A, Hamel CP, Ducommun B, Lenaers G, Belenguer P
FEBS letters 2002 Jul 17;523(1-3):171-6
FEBS letters 2002 Jul 17;523(1-3):171-6
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.
Olichon A, Emorine LJ, Descoins E, Pelloquin L, Brichese L, Gas N, Guillou E, Delettre C, Valette A, Hamel CP, Ducommun B, Lenaers G, Belenguer P
FEBS letters 2002 Jul 17;523(1-3):171-6
FEBS letters 2002 Jul 17;523(1-3):171-6
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
Tang D, Lahti JM, Grenet J, Kidd VJ
The Journal of biological chemistry 1999 Mar 12;274(11):7245-52
The Journal of biological chemistry 1999 Mar 12;274(11):7245-52
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
Tang D, Lahti JM, Grenet J, Kidd VJ
The Journal of biological chemistry 1999 Mar 12;274(11):7245-52
The Journal of biological chemistry 1999 Mar 12;274(11):7245-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of Cytochrome c expression in HeLa cells using Ms x Cytochrome c (Product # 33-8500)
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Cytochrome C Monoclonal Antibody (7H8.2C12) (Product # 33-8500) and a ~15kDa band corresponding to Cytochrome C, along with an characterized band(*) was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), SK-O-V3 (Lane 2) and PC-3 (Lane 3) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
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- Fig. 7. Altered mitochondrial dynamics in Tctp-depleted axons. (A) Ratio of mitochondrial to nuclear DNA determined by qPCR in control and Tctp-depleted retinas. Mean+-95% confidence interval; n =7 paired retinas per condition; P =0.23, Mann-Whitney test. (B) Tctp morphants show unaltered Pgc1alpha expression levels in the CNS as evaluated by western blot analysis of stage 37/38 embryos using an anti-Pgc1alpha antibody. n =3 independent samples; P =0.5955, unpaired t -test. (C) Tctp morphants show unaltered expression of mitochondria-related genes as assessed by RT-qPCR using eye RNA extracts. Mean+-95% confidence interval; n =9 retinas per condition, Mann-Whitney test. (D) Tctp morphants show unaltered cytochrome c expression levels in the CNS, as evaluated by western blot analysis of stage 37/38 embryos using an anti-cytochrome c antibody. n =3 independent samples; P =0.5989, unpaired t -test. (E) Control and Tctp-depleted RGCs have similar levels of cox5a expression. Mean+-s.e.m.; n =~20. GCL, P =0.2026; IPL, P =0.2668; Mann-Whitney test. (F) Representative kymographs (time-space plots) of MitoTracker-labelled RGC axonal mitochondria in control and Tctp morphant backgrounds. The vertical and horizontal axes represent time and spatial position, respectively (e.g. a vertical line indicates a stationary mitochondrion). (G) Summary of changes in axonal mitochondrial dynamics (statistical significance determined using Fisher's exact test). (H) Relative mitochondrial motili
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- Figure 5 Effects of KCN postconditioning on cytochrome c expression in heart tissue. (a) Comparison between the control and KCN groups. * Statistical significance at the level of p < 0.05 between the control group and the KCN group; data are presented as means +- SE. (b) Cytochrome c immunohistochemistry of heart tissues from the control and KCN groups.
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- 10.1371/journal.pone.0258831.g002 Fig 2 Short-term exercise induces weak adaptation of skeletal muscle mitochondria in tumor-free mice. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising or non-exercising tumor-free mice. Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The positive control shown is from the same blot with the intervening lanes cropped out. GAPDH was used as loading control. (B) Densitometric quantification of blots in (A). Samples were normalized to a positive control run on each gel and GAPDH. Activity of citrate synthase (C) and complex IV (D) in quadriceps muscle homogenates from exercising or non-exercising tumor-free mice. Exercise effect: * indicates statistically significantly different from No Ex. Data analyzed by unpaired, two-tailed student's t test (cyt c, CI-III, CV, COX-IV, CS activity and CIV activity) or Mann-Whitney test (CIV expression). p
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- 10.1371/journal.pone.0258831.g003 Fig 3 Exercise increases markers of skeletal muscle mitochondria in untreated and anti-PD-1 treated mice with B16-F10 melanoma, but not mice receiving IgG2a. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising (Ex) or non-exercising (No Ex) mice with B16-F10 melanoma receiving no treatment, an isotype control antibody (IgG2a) or anti-PD-1 (aPD-1). Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The two panels shown represent separate blots, while the dividing lines within panels indicate where lanes have been cropped out. GAPDH was used as loading control. (B) Relative protein expression of cytochrome c, OXPHOS complexes I-V and COX-IV in quadriceps muscle homogenates from exercising or non-exercising B16-F10 melanoma-bearing mice receiving no treatment or treated with anti-PD-1 or an isotype control antibody. Samples were normalized to a positive control (run on all blots) and GAPDH. (C) Activity of citrate synthase in quadriceps muscle homogenates from exercising or non-exercising mice with B16-F10 melanoma receiving no treatment or treated with anti-PD-1 or an isotype control antibody. (D) Activity of complex IV in quadriceps muscle homogenates from exercising or non-exercising mice with B16-F10 melanoma receiving no treatment or treated with anti-PD-1 or an isotype control antibody. *indicate
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- 10.1371/journal.pone.0258831.g004 Fig 4 Exercise induces robust increases in mitochondria marker expression in IgG2a and anti-PD-1-treated, but not untreated, mice with EO771 breast cancer. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising (Ex) or non-exercising (No Ex) mice with EO771 breast cancer receiving no treatment, an isotype control antibody (IgG2a) or anti-PD-1 (aPD-1). Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The two panels shown represent separate blots, while the dividing lines within panels indicate where lanes have been cropped out. (B) Relative protein expression of cytochrome c, OXPHOS complexes I-V and COX-IV in quadriceps muscle homogenates from exercising or non-exercising EO771 breast cancer-bearing mice receiving no treatment or treated with anti-PD-1 or an isotype control antibody. Samples were normalized to a positive control (run on all blots) and GAPDH. (C) Activity of citrate synthase in quadriceps muscle homogenates from exercising or non-exercising mice with EO771 breast cancer receiving no treatment or treated with anti-PD-1 or an isotype control antibody. (D) Activity of complex IV in quadriceps muscle homogenates from exercising or non-exercising mice with EO771 breast cancer receiving no treatment or treated with anti-PD-1 or an isotype control antibody. *indicates statistically significa
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- Figure 3 Chronic influences of exercise on brown adipose tissue at 12-weeks-old. ( A ) Wet weight of the brown adipose tissue; ( B ) Ratio of brown adipose tissue wet weight to body weight; ( C ) Representative section of brown adipose tissue stained with hematoxylin and eosin in the OLETF Sed group; ( D ) Representative sections in the peripheral area of the adipose lobule; ( E ) Diameter of whitened unilocular adipocyte in the central area of the lobule; ( F ) Density of whitened unilocular adipocyte in the central area of the lobule; ( G ) Diameter of hypertrophic unilocular adipocyte in the peripheral area of the lobule; ( H ) Density of hypertrophic unilocular adipocyte in the peripheral area of the lobule; ( I ) Expression levels of the proteins in the brown adipose tissue at 12-weeks-old; ( J ) Representative images for the proteins. OLETF DT, OLETF exercise from 4- to 6-weeks-old and non-exercise detraining from 6- to 12-weeks-old. Arrowheads indicate whitened unilocular adipocytes located in the central area of the adipose lobule. Arrows indicate hypertrophic unilocular adipocytes located in the peripheral area of the adipose lobule. Values represent means +- standard deviation. *, +, and ++ show significantly different from the LETO Sed, OLETF Sed, and OLETF Ex groups, respectively, p < 0.05.