33-8500

antibody from Invitrogen Antibodies

Targeting: CYCS CYC, HCS

Provider product page for 33-8500

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [2]
Other assay [10]

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Product number: 33-8500 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytochrome C Monoclonal Antibody (7H8.2C12)
Antibody type: Monoclonal
Antigen: Other
Reactivity: Human, Mouse, Rat, Drosophila, Rabbit, Xenopus
Host: Mouse
Isotype: IgG
Antibody clone number: 7H8.2C12
Vial size: 200 µg
Concentration: 0.5 mg/mL
Storage: -20°C

CYCS protein structure - 33-8500 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7. Altered mitochondrial dynamics in Tctp-depleted axons. (A) Ratio of mitochondrial to nuclear DNA determined by qPCR in control and Tctp-depleted retinas. Mean+-95% confidence interval; n =7 paired retinas per condition; P =0.23, Mann-Whitney test. (B) Tctp morphants show unaltered Pgc1alpha expression levels in the CNS as evaluated by western blot analysis of stage 37/38 embryos using an anti-Pgc1alpha antibody. n =3 independent samples; P =0.5955, unpaired t -test. (C) Tctp morphants show unaltered expression of mitochondria-related genes as assessed by RT-qPCR using eye RNA extracts. Mean+-95% confidence interval; n =9 retinas per condition, Mann-Whitney test. (D) Tctp morphants show unaltered cytochrome c expression levels in the CNS, as evaluated by western blot analysis of stage 37/38 embryos using an anti-cytochrome c antibody. n =3 independent samples; P =0.5989, unpaired t -test. (E) Control and Tctp-depleted RGCs have similar levels of cox5a expression. Mean+-s.e.m.; n =~20. GCL, P =0.2026; IPL, P =0.2668; Mann-Whitney test. (F) Representative kymographs (time-space plots) of MitoTracker-labelled RGC axonal mitochondria in control and Tctp morphant backgrounds. The vertical and horizontal axes represent time and spatial position, respectively (e.g. a vertical line indicates a stationary mitochondrion). (G) Summary of changes in axonal mitochondrial dynamics (statistical significance determined using Fisher's exact test). (H) Relative mitochondrial motili

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Effects of KCN postconditioning on cytochrome c expression in heart tissue. (a) Comparison between the control and KCN groups. * Statistical significance at the level of p < 0.05 between the control group and the KCN group; data are presented as means +- SE. (b) Cytochrome c immunohistochemistry of heart tissues from the control and KCN groups.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 10.1371/journal.pone.0258831.g002 Fig 2 Short-term exercise induces weak adaptation of skeletal muscle mitochondria in tumor-free mice. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising or non-exercising tumor-free mice. Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The positive control shown is from the same blot with the intervening lanes cropped out. GAPDH was used as loading control. (B) Densitometric quantification of blots in (A). Samples were normalized to a positive control run on each gel and GAPDH. Activity of citrate synthase (C) and complex IV (D) in quadriceps muscle homogenates from exercising or non-exercising tumor-free mice. Exercise effect: * indicates statistically significantly different from No Ex. Data analyzed by unpaired, two-tailed student's t test (cyt c, CI-III, CV, COX-IV, CS activity and CIV activity) or Mann-Whitney test (CIV expression). p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 10.1371/journal.pone.0258831.g003 Fig 3 Exercise increases markers of skeletal muscle mitochondria in untreated and anti-PD-1 treated mice with B16-F10 melanoma, but not mice receiving IgG2a. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising (Ex) or non-exercising (No Ex) mice with B16-F10 melanoma receiving no treatment, an isotype control antibody (IgG2a) or anti-PD-1 (aPD-1). Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The two panels shown represent separate blots, while the dividing lines within panels indicate where lanes have been cropped out. GAPDH was used as loading control. (B) Relative protein expression of cytochrome c, OXPHOS complexes I-V and COX-IV in quadriceps muscle homogenates from exercising or non-exercising B16-F10 melanoma-bearing mice receiving no treatment or treated with anti-PD-1 or an isotype control antibody. Samples were normalized to a positive control (run on all blots) and GAPDH. (C) Activity of citrate synthase in quadriceps muscle homogenates from exercising or non-exercising mice with B16-F10 melanoma receiving no treatment or treated with anti-PD-1 or an isotype control antibody. (D) Activity of complex IV in quadriceps muscle homogenates from exercising or non-exercising mice with B16-F10 melanoma receiving no treatment or treated with anti-PD-1 or an isotype control antibody. *indicate

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1371/journal.pone.0258831.g004 Fig 4 Exercise induces robust increases in mitochondria marker expression in IgG2a and anti-PD-1-treated, but not untreated, mice with EO771 breast cancer. (A) Representative Western blots of quadriceps femoris muscle homogenates from exercising (Ex) or non-exercising (No Ex) mice with EO771 breast cancer receiving no treatment, an isotype control antibody (IgG2a) or anti-PD-1 (aPD-1). Blots were probed for either OXPHOS complexes I-V (CI-V), cytochrome c and GAPDH, or for COX-IV and GAPDH. All samples were run in duplicate on separate gels. The two panels shown represent separate blots, while the dividing lines within panels indicate where lanes have been cropped out. (B) Relative protein expression of cytochrome c, OXPHOS complexes I-V and COX-IV in quadriceps muscle homogenates from exercising or non-exercising EO771 breast cancer-bearing mice receiving no treatment or treated with anti-PD-1 or an isotype control antibody. Samples were normalized to a positive control (run on all blots) and GAPDH. (C) Activity of citrate synthase in quadriceps muscle homogenates from exercising or non-exercising mice with EO771 breast cancer receiving no treatment or treated with anti-PD-1 or an isotype control antibody. (D) Activity of complex IV in quadriceps muscle homogenates from exercising or non-exercising mice with EO771 breast cancer receiving no treatment or treated with anti-PD-1 or an isotype control antibody. *indicates statistically significa

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Chronic influences of exercise on brown adipose tissue at 12-weeks-old. ( A ) Wet weight of the brown adipose tissue; ( B ) Ratio of brown adipose tissue wet weight to body weight; ( C ) Representative section of brown adipose tissue stained with hematoxylin and eosin in the OLETF Sed group; ( D ) Representative sections in the peripheral area of the adipose lobule; ( E ) Diameter of whitened unilocular adipocyte in the central area of the lobule; ( F ) Density of whitened unilocular adipocyte in the central area of the lobule; ( G ) Diameter of hypertrophic unilocular adipocyte in the peripheral area of the lobule; ( H ) Density of hypertrophic unilocular adipocyte in the peripheral area of the lobule; ( I ) Expression levels of the proteins in the brown adipose tissue at 12-weeks-old; ( J ) Representative images for the proteins. OLETF DT, OLETF exercise from 4- to 6-weeks-old and non-exercise detraining from 6- to 12-weeks-old. Arrowheads indicate whitened unilocular adipocytes located in the central area of the adipose lobule. Arrows indicate hypertrophic unilocular adipocytes located in the peripheral area of the adipose lobule. Values represent means +- standard deviation. *, +, and ++ show significantly different from the LETO Sed, OLETF Sed, and OLETF Ex groups, respectively, p < 0.05.