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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- V2786 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cytochrome C Antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Canine, Horse, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 7H8.2C12
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana.
Effects of Nitric Oxide Synthase Inhibition on Fiber-Type Composition, Mitochondrial Biogenesis, and SIRT1 Expression in Rat Skeletal Muscle.
Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis.
Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells.
Capillin, a major constituent of Artemisia capillaris Thunb. flower essential oil, induces apoptosis through the mitochondrial pathway in human leukemia HL-60 cells.
Mcl-1 antagonism is a potential therapeutic strategy in a subset of solid cancers.
Cytochrome c oxidase deficiency accelerates mitochondrial apoptosis by activating ceramide synthase 6.
Macrophage mitochondrial and stress response to ingestion of Cryptococcus neoformans.
A comparison of chronic AICAR treatment-induced metabolic adaptations in red and white muscles of rats.
MCL-1 Is a Key Determinant of Breast Cancer Cell Survival: Validation of MCL-1 Dependency Utilizing a Highly Selective Small Molecule Inhibitor.
c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway.
Reape TJ, Kacprzyk J, Brogan N, Sweetlove L, McCabe PF
Methods in molecular biology (Clifton, N.J.) 2015;1305:211-21
Methods in molecular biology (Clifton, N.J.) 2015;1305:211-21
Effects of Nitric Oxide Synthase Inhibition on Fiber-Type Composition, Mitochondrial Biogenesis, and SIRT1 Expression in Rat Skeletal Muscle.
Suwa M, Nakano H, Radak Z, Kumagai S
Journal of sports science & medicine 2015 Sep;14(3):548-55
Journal of sports science & medicine 2015 Sep;14(3):548-55
Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis.
Seko Y, Fujimura T, Yao T, Taka H, Mineki R, Okumura K, Murayama K
Scientific reports 2015 Sep 8;5:13737
Scientific reports 2015 Sep 8;5:13737
Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells.
Joo JH, Ueda E, Bortner CD, Yang XP, Liao G, Jetten AM
Biochemical pharmacology 2015 Oct 1;97(3):256-68
Biochemical pharmacology 2015 Oct 1;97(3):256-68
Capillin, a major constituent of Artemisia capillaris Thunb. flower essential oil, induces apoptosis through the mitochondrial pathway in human leukemia HL-60 cells.
Masuda Y, Asada K, Satoh R, Takada K, Kitajima J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2015 May 15;22(5):545-52
Phytomedicine : international journal of phytotherapy and phytopharmacology 2015 May 15;22(5):545-52
Mcl-1 antagonism is a potential therapeutic strategy in a subset of solid cancers.
Modugno M, Banfi P, Gasparri F, Borzilleri R, Carter P, Cornelius L, Gottardis M, Lee V, Mapelli C, Naglich JG, Tebben A, Vite G, Pastori W, Albanese C, Corti E, Ballinari D, Galvani A
Experimental cell research 2015 Mar 15;332(2):267-77
Experimental cell research 2015 Mar 15;332(2):267-77
Cytochrome c oxidase deficiency accelerates mitochondrial apoptosis by activating ceramide synthase 6.
Schüll S, Günther SD, Brodesser S, Seeger JM, Tosetti B, Wiegmann K, Pongratz C, Diaz F, Witt A, Andree M, Brinkmann K, Krönke M, Wiesner RJ, Kashkar H
Cell death & disease 2015 Mar 12;6:e1691
Cell death & disease 2015 Mar 12;6:e1691
Macrophage mitochondrial and stress response to ingestion of Cryptococcus neoformans.
Coelho C, Souza AC, Derengowski Lda S, de Leon-Rodriguez C, Wang B, Leon-Rivera R, Bocca AL, Gonçalves T, Casadevall A
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2345-57
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2345-57
A comparison of chronic AICAR treatment-induced metabolic adaptations in red and white muscles of rats.
Suwa M, Nakano H, Radak Z, Kumagai S
The journal of physiological sciences : JPS 2015 Jan;65(1):121-30
The journal of physiological sciences : JPS 2015 Jan;65(1):121-30
MCL-1 Is a Key Determinant of Breast Cancer Cell Survival: Validation of MCL-1 Dependency Utilizing a Highly Selective Small Molecule Inhibitor.
Xiao Y, Nimmer P, Sheppard GS, Bruncko M, Hessler P, Lu X, Roberts-Rapp L, Pappano WN, Elmore SW, Souers AJ, Leverson JD, Phillips DC
Molecular cancer therapeutics 2015 Aug;14(8):1837-47
Molecular cancer therapeutics 2015 Aug;14(8):1837-47
c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway.
He MX, He YW
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 1;194(7):3444-51
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 1;194(7):3444-51
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HL-60 cell lysate with Cytochrome C antibody (clone 7H8.2C12). Predicted molecular weight: ~12kDa, routinely visualized at ~15kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human salivary tumor stained with Cytochrome C antibody (7H8.2C12).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human pancreas stained with Cytochrome C antibody (7H8.2C12).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Cytochrome C Antibody (clone 7H8.2C12). Confirmation of Integrity and Purity of the Antibody.
