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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [39]
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- Product number
- NB100-55775 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55775, RRID:AB_838083
- Product name
- Mouse Monoclonal Cytochrome c Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- Reactivity
- Human, Mouse, Rat, Drosophila, Rabbit
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice.
Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines.
Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction.
Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1.
Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release.
A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis.
A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis.
Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL.
Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences.
Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia.
Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation.
Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation.
In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.
Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients.
Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients.
Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli.
Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia.
Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia.
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity.
Early mitochondrial activation and cytochrome c up-regulation during apoptosis.
Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells.
Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells.
Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases.
Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases.
Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target.
Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target.
Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore.
Metallothionein inhibits doxorubicin-induced mitochondrial cytochrome c release and caspase-3 activation in cardiomyocytes.
Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors.
XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage.
Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells.
Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis.
Purified photoproducts of merocyanine 540 trigger cytochrome C release and caspase 8-dependent apoptosis in human leukemia and melanoma cells.
Blocking cytochrome c activity within intact neurons inhibits apoptosis.
Blocking cytochrome c activity within intact neurons inhibits apoptosis.
Cytosolic redistribution of cytochrome c after transient focal cerebral ischemia in rats.
A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells.
Csak T, Dolganiuc A, Kodys K, Nath B, Petrasek J, Bala S, Lippai D, Szabo G
Hepatology (Baltimore, Md.) 2011 Jun;53(6):1917-31
Hepatology (Baltimore, Md.) 2011 Jun;53(6):1917-31
Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines.
Kamal A, Sreekanth K, Kumar PP, Shankaraiah N, Balakishan G, Ramaiah MJ, Pushpavalli SN, Ray P, Bhadra MP
European journal of medicinal chemistry 2010 Jun;45(6):2173-81
European journal of medicinal chemistry 2010 Jun;45(6):2173-81
Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction.
Douglas RM, Ryu J, Kanaan A, Del Carmen Rivero M, Dugan LL, Haddad GG, Ali SS
American journal of physiology. Cell physiology 2010 Jun;298(6):C1594-602
American journal of physiology. Cell physiology 2010 Jun;298(6):C1594-602
Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1.
Zall H, Weber A, Besch R, Zantl N, Häcker G
Molecular cancer 2010 Jun 24;9:164
Molecular cancer 2010 Jun 24;9:164
Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release.
Kumar P, Coltas IK, Kumar B, Chepeha DB, Bradford CR, Polverini PJ
Cancer research 2007 Feb 1;67(3):1193-202
Cancer research 2007 Feb 1;67(3):1193-202
A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis.
Chen M, Gan H, Remold HG
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3707-16
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3707-16
A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis.
Chen M, Gan H, Remold HG
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3707-16
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3707-16
Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL.
Meyer LH, Karawajew L, Schrappe M, Ludwig WD, Debatin KM, Stahnke K
Blood 2006 Jun 1;107(11):4524-31
Blood 2006 Jun 1;107(11):4524-31
Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences.
De Chiara G, Marcocci ME, Torcia M, Lucibello M, Rosini P, Bonini P, Higashimoto Y, Damonte G, Armirotti A, Amodei S, Palamara AT, Russo T, Garaci E, Cozzolino F
The Journal of biological chemistry 2006 Jul 28;281(30):21353-61
The Journal of biological chemistry 2006 Jul 28;281(30):21353-61
Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia.
Zhu C, Xu F, Wang X, Shibata M, Uchiyama Y, Blomgren K, Hagberg H
Journal of neurochemistry 2006 Feb;96(4):1016-27
Journal of neurochemistry 2006 Feb;96(4):1016-27
Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation.
Yamasaki E, Wada A, Kumatori A, Nakagawa I, Funao J, Nakayama M, Hisatsune J, Kimura M, Moss J, Hirayama T
The Journal of biological chemistry 2006 Apr 21;281(16):11250-9
The Journal of biological chemistry 2006 Apr 21;281(16):11250-9
Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation.
Arnoult D, Grodet A, Lee YJ, Estaquier J, Blackstone C
The Journal of biological chemistry 2005 Oct 21;280(42):35742-50
The Journal of biological chemistry 2005 Oct 21;280(42):35742-50
In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.
Wong SL, Chen Y, Chan CM, Chan CS, Chan PK, Chui YL, Fung KP, Waye MM, Tsui SK, Chan HY
Biochemical and biophysical research communications 2005 Nov 18;337(2):720-9
Biochemical and biophysical research communications 2005 Nov 18;337(2):720-9
Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients.
Boden G, Homko C, Mozzoli M, Showe LC, Nichols C, Cheung P
Diabetes 2005 Mar;54(3):880-5
Diabetes 2005 Mar;54(3):880-5
Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients.
Boden G, Homko C, Mozzoli M, Showe LC, Nichols C, Cheung P
Diabetes 2005 Mar;54(3):880-5
Diabetes 2005 Mar;54(3):880-5
Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli.
Liang Y, Eid MA, Lewis RW, Kumar MV
Cellular signalling 2005 Feb;17(2):243-51
Cellular signalling 2005 Feb;17(2):243-51
Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia.
Zhu C, Wang X, Cheng X, Qiu L, Xu F, Simbruner G, Blomgren K
Brain research 2004 Jan 16;996(1):67-75
Brain research 2004 Jan 16;996(1):67-75
Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia.
Zhu C, Wang X, Cheng X, Qiu L, Xu F, Simbruner G, Blomgren K
Brain research 2004 Jan 16;996(1):67-75
Brain research 2004 Jan 16;996(1):67-75
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Liu CY, Lee CF, Hong CH, Wei YH
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Liu CY, Lee CF, Hong CH, Wei YH
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity.
Colell A, García-Ruiz C, Lluis JM, Coll O, Mari M, Fernández-Checa JC
The Journal of biological chemistry 2003 Sep 5;278(36):33928-35
The Journal of biological chemistry 2003 Sep 5;278(36):33928-35
Early mitochondrial activation and cytochrome c up-regulation during apoptosis.
Chandra D, Liu JW, Tang DG
The Journal of biological chemistry 2002 Dec 27;277(52):50842-54
The Journal of biological chemistry 2002 Dec 27;277(52):50842-54
Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells.
Bitzer M, Armeanu S, Prinz F, Ungerechts G, Wybranietz W, Spiegel M, Bernlöhr C, Cecconi F, Gregor M, Neubert WJ, Schulze-Osthoff K, Lauer UM
The Journal of biological chemistry 2002 Aug 16;277(33):29817-24
The Journal of biological chemistry 2002 Aug 16;277(33):29817-24
Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells.
Narvaez CJ, Welsh J
The Journal of biological chemistry 2001 Mar 23;276(12):9101-7
The Journal of biological chemistry 2001 Mar 23;276(12):9101-7
Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases.
Junn E, Mouradian MM
Journal of neurochemistry 2001 Jul;78(2):374-83
Journal of neurochemistry 2001 Jul;78(2):374-83
Apoptotic signaling in dopamine-induced cell death: the role of oxidative stress, p38 mitogen-activated protein kinase, cytochrome c and caspases.
Junn E, Mouradian MM
Journal of neurochemistry 2001 Jul;78(2):374-83
Journal of neurochemistry 2001 Jul;78(2):374-83
Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target.
Shen HM, Yang CF, Ding WX, Liu J, Ong CN
Free radical biology & medicine 2001 Jan 1;30(1):9-21
Free radical biology & medicine 2001 Jan 1;30(1):9-21
Superoxide radical-initiated apoptotic signalling pathway in selenite-treated HepG(2) cells: mitochondria serve as the main target.
Shen HM, Yang CF, Ding WX, Liu J, Ong CN
Free radical biology & medicine 2001 Jan 1;30(1):9-21
Free radical biology & medicine 2001 Jan 1;30(1):9-21
Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore.
Takuma K, Phuagphong P, Lee E, Mori K, Baba A, Matsuda T
The Journal of biological chemistry 2001 Dec 21;276(51):48093-9
The Journal of biological chemistry 2001 Dec 21;276(51):48093-9
Metallothionein inhibits doxorubicin-induced mitochondrial cytochrome c release and caspase-3 activation in cardiomyocytes.
Wang GW, Klein JB, Kang YJ
The Journal of pharmacology and experimental therapeutics 2001 Aug;298(2):461-8
The Journal of pharmacology and experimental therapeutics 2001 Aug;298(2):461-8
Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors.
Maccarrone M, Lorenzon T, Bari M, Melino G, Finazzi-Agro A
The Journal of biological chemistry 2000 Oct 13;275(41):31938-45
The Journal of biological chemistry 2000 Oct 13;275(41):31938-45
XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage.
Datta R, Oki E, Endo K, Biedermann V, Ren J, Kufe D
The Journal of biological chemistry 2000 Oct 13;275(41):31733-8
The Journal of biological chemistry 2000 Oct 13;275(41):31733-8
Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells.
Walczak H, Bouchon A, Stahl H, Krammer PH
Cancer research 2000 Jun 1;60(11):3051-7
Cancer research 2000 Jun 1;60(11):3051-7
Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis.
Maeno E, Ishizaki Y, Kanaseki T, Hazama A, Okada Y
Proceedings of the National Academy of Sciences of the United States of America 2000 Aug 15;97(17):9487-92
Proceedings of the National Academy of Sciences of the United States of America 2000 Aug 15;97(17):9487-92
Purified photoproducts of merocyanine 540 trigger cytochrome C release and caspase 8-dependent apoptosis in human leukemia and melanoma cells.
Pervaiz S, Seyed MA, Hirpara JL, Clément MV, Loh KW
Blood 1999 Jun 15;93(12):4096-108
Blood 1999 Jun 15;93(12):4096-108
Blocking cytochrome c activity within intact neurons inhibits apoptosis.
Neame SJ, Rubin LL, Philpott KL
The Journal of cell biology 1998 Sep 21;142(6):1583-93
The Journal of cell biology 1998 Sep 21;142(6):1583-93
Blocking cytochrome c activity within intact neurons inhibits apoptosis.
Neame SJ, Rubin LL, Philpott KL
The Journal of cell biology 1998 Sep 21;142(6):1583-93
The Journal of cell biology 1998 Sep 21;142(6):1583-93
Cytosolic redistribution of cytochrome c after transient focal cerebral ischemia in rats.
Fujimura M, Morita-Fujimura Y, Murakami K, Kawase M, Chan PH
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 1998 Nov;18(11):1239-47
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 1998 Nov;18(11):1239-47
A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells.
Jemmerson R, Johnson JG, Burrell E, Taylor PS, Jenkins MK
European journal of immunology 1991 Jan;21(1):143-51
European journal of immunology 1991 Jan;21(1):143-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome C Antibody (7H8.2C12) [Biotin] [NB100-55775] - Western blot detection of Cytochrome C in 15 mgs of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.
