11-6601-82

antibody from Invitrogen Antibodies

Targeting: CYCS CYC, HCS

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11-6601-82

Provider

Invitrogen Antibodies

Product name

Cytochrome C Monoclonal Antibody (6H2), FITC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c. Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

6H2

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis.

Cooley JC, Javkhlan N, Wilson JA, Foster DG, Edelman BL, Ortiz LA, Schwartz DA, Riches DW, Redente EF
JCI insight 2023 Feb 8;8(3)

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Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Moretti FA, Giardino G, Attenborough TCH, Gkazi AS, Margetts BK, la Marca G, Fairbanks L, Crompton T, Gaspar HB
Scientific reports 2021 Dec 1;11(1):23221

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Caspase dependent programmed cell death in developing embryos: a potential target for therapeutic intervention against pathogenic nematodes.

Mohapatra AD, Kumar S, Satapathy AK, Ravindran B
PLoS neglected tropical diseases 2011 Sep;5(9):e1306

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Cytochrome C was performed using log phase HeLa cells treated with 50 uM of Etoposide for 3 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome C, FITC conjugated Monoclonal Antibody (Product # 11-6601-82) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Mitochondria was stained with MitoTracker® Red CMXRos (Product # M7512). Panel d represents the merged image showing cytoplasmic release of Cytochrome C from mitochondria on etoposide treatment. Panel e represents untreated cells showing mitochondrial localization. Panel f represents FITC Isotype control cells to assess background. The images were captured at 60X magnification.

Conjugate

Green dye

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of the HeLa cell line with anti-Cytochrome c FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of the HeLa cell line with anti-Cytochrome c FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Demonstration of Cytochrome-c-CED-4 interaction in S.digitata and docking of Cytochrome-c and CED-4 of C.elegans. [ A ] Confocal images of untreated control [upper panel] or Plumbagin treated [lower panel] late embryonic stages demonstrating enhanced colocalization of Cytochrome-c and CED-4 after 24 hr Plumbagin treatment are shown. Regions of colocalization are highlighted as white patches using Image J software. Fluorescence intensity line profile/colocalization profile analysis for both the labeled proteins-Cytochrome-c and CED-4 in control as well as apoptotic embryos, revealing enhanced cololocalization of these proteins in the later are shown [ B ] Interaction of Cytochrome-c of C.elegans [ Magenta ] with alpha/beta [P-loop] ATP binding domain [ Blue ] of CED-4 is shown.

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Immature thymocytes from ADA-deficient mice show increased expression of apoptotic markers. ( A ) Western blot analysis of protein lysates of thymi from P14 untreated mice (lane 1-6) and 3-month-PEG-ADA-treated mice (lane 7-8). As positive control for cleaved caspase-3 staining, unfractionated thymocytes were stimulated in vitro with the apoptosis-inducer etoposide (line 9). Anti-GAPDH stain was used as protein loading control. Full-length blots are presented in Supplementary Fig. 11 . ( B ) FACS analysis of MACS-enriched Lin neg thymocytes from untreated mice at P14. DN4 (Lin neg , CD25 - ) and DN2 + DN3 (Lin neg , CD25 + ) populations are gated. ( C ) Intracellular staining for cleaved caspase-3 of cell populations shown in (B). Unfractionated Lin neg thymocytes were stimulated in vitro with etoposide as apoptosis positive control. ( D ) Intracellular staining for cytochrome C of cell populations shown in (B). FACS plots in (C) and (D) are representative of two replicate experiments (n = 4, 2).

Conjugate

Green dye