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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V8015 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Cytochrome C Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Cytochrome C antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- CYCS/3128R
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human heart lysate with recombinant Cytochrome C antibody. Predicted molecular weight: 12-14 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant Cytochrome C antibody (clone CYCS/3128R, green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver with recombinant Cytochrome C antibody (clone CYCS/3128R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytochrome C antibody (clone CYCS/3128R); Red=isotype control, Blue= recombinant Cytochrome C antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Cytochrome C antibody (clone CYCS/3128R) as confirmation of integrity and purity.
