Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- Ab098695 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Cytochrome C Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Cytochrome C Antibody, Monoclonal (C2), could be used for WB,IHC,ICC,IF and so on. Application: WB: 0.01-2µg/mL IHC: 5-30µg/mL ICC/IF: 5-30µg/mL Protein Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 5-100
- Antibody clone number
- C2
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - Western Blot All lanes: Cytochrome C Mouse mAb (Ab098695) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 12 kDa Observed band size: 16 kDa Exposure time: 7.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IF/ICC FITC staining on IF Sample: Human Hep G2 cell Primary Ab: 20 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IF/ICC FITC staining on IF Sample: Human HeLa cell Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Cerebellum Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Cardiac Muscle Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Small intestine Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Lymph node Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.