PA5-28856

antibody from Invitrogen Antibodies

Targeting: CYCS CYC, HCS

Antibody data

Submit

Validation data

Product number: PA5-28856 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytochrome C Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: 293T, K562, THP-1, HL-60, Mouse brain, PC-12, CYCS shRNA-transfected 293T, 293T, SCC-25. Predicted reactivity: Mouse (91%), Rat (91%), Xenopus laevis (85%), Dog (89%), Pig (90%), Chicken (87%), Chimpanzee (100%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.18 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CYCS protein structure - PA5-28856 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cytochrome C using 30 µg of K562 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Cytochrome C polyclonal antibody (Product # PA5-28856) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Cytochrome C was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cytochrome C Polyclonal Antibody (Product # PA5-28856) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Cytochrome C was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cytochrome C Polyclonal Antibody (Product # PA5-28856) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cytochrome C Polyclonal Antibody (Product # PA5-28856). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 15% SDS PAGE. Cytochrome C Polyclonal Antibody (Product # PA5-28856) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cytochrome C Polyclonal Antibody (Product # PA5-28856). HepG2 and mitochondria extracts (30 µg) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C Polyclonal Antibody (Product # PA5-28856) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Cytochrome C Polyclonal Antibody (Product # PA5-28856). Sample (30 µg of whole cell lysate). Lane A: PC-12. 15% SDS PAGE. Cytochrome C Polyclonal Antibody (Product # PA5-28856) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.