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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- MAB898 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Equine Cytochrome c Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects native human Cytochrome c but not mouse Cytochrome c.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2B5F8
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma.
Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD Jr
Molecular cancer therapeutics 2008 Mar;7(3):500-9
Molecular cancer therapeutics 2008 Mar;7(3):500-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytochrome c in Human PBMCs. Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immunoprecipitated from lysates (5 x 106 cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complex-es were absorbed using Protein A Immunoprecipitin (Life Tech-nologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.
