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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- MAB899 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Cytochrome c Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects native human, mouse, and rat Cytochrome c.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2.7D5
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immuno-precipitated from lysates (5 x 106 cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB899) for 1 hour at 4 °C. Cytochrome c-antibody comp-lexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecip-itated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Mouse Anti-Human/ Mouse/Rat Cytochrome c Monoclonal Antibody (Cata-log # MAB897). View our recommended buffer recipes for immunoprecipitation.
