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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501461 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501461, RRID:AB_11128605
- Product name
- EFNA2 (Ephrin A2) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
- Antibody type
- Monoclonal
- Description
- EFNA2 (Ephrin A2) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EFNA2
- Isotype
- IgG
- Antibody clone number
- OTI3E3
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (RC213728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-EFNA2 mouse monoclonal antibody (TA501461) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2(RC213728).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501461)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody(TA501461),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
